Acute administration of the olive constituent, oleuropein, combined with ischemic postconditioning increases myocardial protection by modulating oxidative defense

Oleuropein, one of the main polyphenolic constituents olive, is cardioprotective against ischemia reperfusion injury (IRI). We aimed to assess cardioprotection afforded by acute administration oleuropein and evaluate underlying mechanism. Importantly, since antioxidant therapies have yielded inconclusive results in attenuating IRI-induced damage on top conditioning strategies, we investigated whether could enhance or imbed manifestation ischemic postconditioning (PostC). given during as a single intravenous bolus dose reduced infarct size compared control group both rabbits mice subjected myocardial IRI. None inhibitors pathways, l-NAME, wortmannin AG490, influence its limiting effects. Combined PostC cause further limitation comparison with alone animal models. Oleuropein did not inhibit calcium induced mitochondrial permeability transition pore opening isolated mitochondria increase cGMP production. To provide insights different mechanism oleuropein, sought characterize anti-inflammatory potential vivo. their combination reduce inflammatory monocytes infiltration into heart circulating monocyte cell population. Oleuropein's action involves direct protective effect cardiomyocytes it significantly increased viability following simulated IRI non-treated cells. ?leuropein confers additive PostC, via increasing expression transcription factor Nrf-2 downstream targets In conclusion, provides robust synergistic experimental models inducing defense genes through axis independently classic signaling pathways (RISK, cGMP/PKG, SAFE).